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KMID : 1094720190240040666
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Biotechnology and Bioprocess Engineering
2019 Volume.24 No. 4 p.666 ~ p.674
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Preparation of High-quality Glabridin Extract from Glycyrrhiza glabra
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Hong Jin-Hee
Jung In-Il
Cho Yun-Kyoung
Haam Seung-Joo
Lee Sang-Yun
Lim Gio-Bin
Ryu Jong-Hoon
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Abstract
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Supercritical fluid extraction (SFE) using cosolvent-modified supercritical carbon dioxide and a two-step separation/purification method was investigated as a way to improve the purity of glabridin, one of the many bioactive components of Glycyrrhiza glabra (licorice). The SFE parameters were optimized using an analytical-scale SFE system in the temperature range 40?80°C and the pressure range 10?50 MPa. The extraction was then scaled up by 100 times using a preparative SFE system under the following set of optimized conditions: 40°C, 30 MPa, and SCCO2 modified with ethanol equivalent to its 25% (v/v) concentration. The glabridin purity obtained through the scaled-up SFE system was 6.2%, a much higher level than that obtained through organic solvent extraction. The licorice extract obtained by scaling up the SFE system was isolated and purified by applying alcohol precipitation/ filtration and adsorption chromatography with 80% aqueous ethanol to obtain a purer product. It was confirmed that the glabridin purity of the final extract product was increased by up to 37%, without significant loss of glabridin after two separation/purification steps.
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KEYWORD
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supercritical fluid extraction, glabridin, Glycyrrhiza glabra, purification
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